Excretion of Mycobacterium bovis by experimentally infected cattle

Veterinary Research Laboratories, Stormont, Belfast.

Three groups, each of five calves, four to seven months old, were inoculated intranasally with different numbers of Mycobacterium bovis. Infection was established readily in the calves which received an inoculum containing either 10(6) or 10(4) colony forming units (cfu). After every infection there was a lag period during which the organisms could not be isolated from specimens of nasal mucus. All the animals excreted M bovis and the time of commencement, quantity and duration of excretion appeared to be related to the inoculation dose. Excretion continued for many weeks, and for two calves excretion became intermittent over many months. All the calves which were given inocula of 92 cfu failed to develop the disease and no immunological responses were detected; however, M bovis was isolated from nasal secretions from one of these animals 100 days after inoculation.

This article has been cited by other articles:

				W. R. Waters, M. V. Palmer, T. C. Thacker, J. P. Bannantine, H. M. Vordermeier, R. G. Hewinson, R. Greenwald, J. Esfandiari, J. McNair, J. M. Pollock, et al. Early Antibody Responses to Experimental Mycobacterium bovis Infection of Cattle Clin. Vaccine Immunol., June 1, 2006; 13(6): 648 - 654. [Abstract] [Full Text] [PDF]

				G. S. Dean, S. G. Rhodes, M. Coad, A. O. Whelan, P. J. Cockle, D. J. Clifford, R. G. Hewinson, and H. M. Vordermeier Minimum Infective Dose of Mycobacterium bovis in Cattle Infect. Immun., October 1, 2005; 73(10): 6467 - 6471. [Abstract] [Full Text] [PDF]

				C. Aagaard, M. Govaerts, L. Meng Okkels, P. Andersen, and J. M. Pollock Genomic Approach to Identification of Mycobacterium bovis Diagnostic Antigens in Cattle J. Clin. Microbiol., August 1, 2003; 41(8): 3719 - 3728. [Abstract] [Full Text] [PDF]

				J. M. Pollock, J. McNair, H. Bassett, J. P. Cassidy, E. Costello, H. Aggerbeck, I. Rosenkrands, and P. Andersen Specific Delayed-Type Hypersensitivity Responses to ESAT-6 Identify Tuberculosis-Infected Cattle J. Clin. Microbiol., May 1, 2003; 41(5): 1856 - 1860. [Abstract] [Full Text] [PDF]

				M. D. Welsh, H. E. Kennedy, A. J. Smyth, R. M. Girvin, P. Andersen, and J. M. Pollock Responses of Bovine WC1+{gamma}{delta} T Cells to Protein and Nonprotein Antigens of Mycobacterium bovis Infect. Immun., November 1, 2002; 70(11): 6114 - 6120. [Abstract] [Full Text] [PDF]

				H. E. Kennedy, M. D. Welsh, D. G. Bryson, J. P. Cassidy, F. I. Forster, C. J. Howard, R. A. Collins, and J. M. Pollock Modulation of Immune Responses to Mycobacterium bovis in Cattle Depleted of WC1+{gamma}{delta} T Cells Infect. Immun., March 1, 2002; 70(3): 1488 - 1500. [Abstract] [Full Text] [PDF]

				A. J. Smyth, M. D. Welsh, R. M. Girvin, and J. M. Pollock In Vitro Responsiveness of {gamma}{delta} T Cells from Mycobacterium bovis-Infected Cattle to Mycobacterial Antigens: Predominant Involvement of WC1+ Cells Infect. Immun., January 1, 2001; 69(1): 89 - 96. [Abstract] [Full Text] [PDF]

				L. A. H. van Pinxteren, P. Ravn, E. M. Agger, J. Pollock, and P. Andersen Diagnosis of Tuberculosis Based on the Two Specific Antigens ESAT-6 and CFP10 Clin. Vaccine Immunol., March 1, 2000; 7(2): 155 - 160. [Abstract] [Full Text] [PDF]

				E. Liebana, R. M. Girvin, M. Welsh, S. D. Neill, and J. M. Pollock Generation of CD8+ T-Cell Responses to Mycobacterium bovis and Mycobacterial Antigen in Experimental Bovine Tuberculosis Infect. Immun., March 1, 1999; 67(3): 1034 - 1044. [Abstract] [Full Text] [PDF]

				K. P. Lyashchenko, J. M. Pollock, R. Colangeli, and M. L. Gennaro Diversity of Antigen Recognition by Serum Antibodies in Experimental Bovine Tuberculosis Infect. Immun., November 1, 1998; 66(11): 5344 - 5349. [Abstract] [Full Text] [PDF]