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The Veterinary Record, Vol 139, Issue 21, 515-519
Copyright © 1996 by British Veterinary Association

Immunohistochemical method for detecting lesions in the prostate gland of bulls treated with diethylstilbestrol-dipropionate

J. Weijman DVM1, P. Zwart DVM, PhD1, J. G. Vos DVM, PhD1, and F. C. S. Ramaekers PhD2

1 Department of Veterinary Pathology, Utrecht University, Yalelaan 1, PO Box 80.158, 3508 TD Utrecht, The Netherlands
2 Department of Molecular Cell Biology and Genetics, University of Limburg, Maastricht, The Netherlands

Hyperplastic and metaplastic lesions in the prostate gland of bulls are used as morphological evidence for the illegal administration of oestrogens or compounds with oestrogenic activity, like stilbenes. Exogenous and endogenous androgens can suppress the effects of these drugs, resulting in suspect or negative results. The detection of epithelial changes immunohistochemically with cytokeratin antibodies appeared to facilitate the diagnosis of hyperplasia and metaplasia in the prostate. A specific and sensitive method for detecting such lesions in formalin-fixed, paraffin-embedded prostate samples has been developed with the commercially available cytokeratin monoclonal antibody 34betaE12, and applied to bulls treated with diethylstilbestrol-dipropionate. The highly reproducible staining pattern of 34betaE12 can be used for the detection of hyperplasia and metaplasia in the prostate glands of animals treated illegally with oestrogens.






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Copyright © 1996 British Veterinary Association