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The Veterinary Record, Vol 140, Issue 12, 310-313
Copyright © 1997 by British Veterinary Association

Comparison of Chlamydia psittaci from cats with upper respiratory tract disease by polymerase chain reaction analysis of the ompA gene

J. E. Sykes BVSc1, G. F. Browning BVSc, DipVetClinStud, PhD1, V. P. Studdert BS, DVM2, and G. Anderson BAgrSci, GradDipApplStats2

1 The University of Melbourne, Department of Veterinary Science, Veterinary Preclinical Centre, corner of Flemington Road and Park Drive, Parkville, Victoria 3052, Australia
2 The University of Melbourne, Veterinary Clinical Centre, 250 Princes Highway, Werribee, Victoria 3030, Australia

Conjunctival swabs were taken from 168 cats with clinical signs of acute or chronic upper respiratory tract disease and tested for Chlamydia psittaci by the polymerase chain reaction (PCR) to amplify the ompA gene coding region. Twenty-two (13 per cent) were positive for C psittaci. The PCR products from positive samples were subjected to restriction endonuclease analysis with the restriction enzymes Alu I and Mse I. The fragments of DNA were detected on silver-stained polyacrylamide gels and the results were compared with the results obtained from chlamydial isolates from cats in Japan, France, the USA and the UK. All the strains had identical restriction patterns. When PCR is used as an epidemiological tool, feline chlamydial strains worldwide appear very similar






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Copyright © 1997 British Veterinary Association