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The Veterinary Record 159:742-745 (2006)
© 2006 British Veterinary Association

Papers and Articles

Field evaluation of a PCR for the diagnosis of chlamydial abortion in sheep

L. Güler, DVM, PhD1, H. H. Hadimli, DVM, PhD1, O. Erganis, DVM, PhD1, M. Ates, DVM, PhD1, Ü. Ok, DVM, PhD2 and K. Gündüz, DVM, PhD1

1 Department of Microbiology, Faculty of Veterinary Medicine, Selcuk University, 42020 Konya, Turkey
2 Veterinary Control and Research Institute, 42080 Konya, Turkey

A total of 94 vaginal swab samples and 195 serum samples collected from aborted ewes in 15 flocks were examined by PCR and a complement fixation test, respectively. In addition, 172 samples of stomach contents from fetuses from different flocks submitted for the diagnosis of abortion during the four lambing periods between 2000 and 2004 were tested by PCR. Chlamydial DNA was detected in seven vaginal swabs obtained from five of the 15 flocks and in six samples of fetal stomach contents. The results of PCR and flock serology for Chlamydia were positive in five of the 15 flocks and negative in eight.






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