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Sensitive test for screening for Staphylococcus aureus in bovine mastitis by broth cultivation and PCR

¹ Research Unit for Food Animal Medicine, Research Centre for Animal Hygiene and Food Safety, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan
² Research Unit for Foodborne Diseases, Research Centre for Animal Hygiene and Food Safety, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan
³ Research Unit for Veterinary Microbiology and Food Safety, Department of Veterinary Medicine, Faculty of Agriculture, Iwate University, Morioka, Iwate 020-8550, Japan
⁴ Research Unit for Diagnosis and Treatment of Food Animal Diseases, Department of Veterinary Medicine, Faculty of Agriculture, Iwate University, Morioka, Iwate 020-8550, Japan
⁵ Hokubu Livestock Hygiene Service Centre, Ishikawa Prefecture, 1-47 Otsu Nanao, Ishikawa 929-2126, Japan

An assay was developed and evaluated for screening for Staphylococcus aureus in milk samples from cases of bovine mastitis by overnight cultivation in a broth containing 7·5 per cent sodium chloride, followed by PCR to amplify the nuc gene. The assay could detect concentrations of S aureus as low as 1 colony-forming unit/ml milk. Among 106 milk samples collected from individual quarters of lactating cows in one dairy herd and from a bulk tank, S aureus was detected in nine samples by the PCR assay but in only three samples by conventional microbiological culture.

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