HOME CURRENT ISSUE TABLE OF CONTENTS ARCHIVE SEARCH SUBSCRIPTIONS JOBS FEEDBACK HELP		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ameni, G. Articles by Gordon, S. V. Search for Related Content PubMed PubMed Citation Articles by Ameni, G. Articles by Gordon, S. V.

Effect of skin testing and segregation on the prevalence of bovine tuberculosis, and molecular typing of Mycobacterium bovis, in Ethiopia

¹ Aklilu Lemma Institute of Pathobiology, Addis Ababa University, PO Box 1176, Addis Ababa, Ethiopia
² Armauer Hansen Research Institute, PO Box 1005, Addis Ababa, Ethiopia
³ National Animal Health Research Centre, Ethiopian Agricultural Research Organisation, Sebeta, Ethiopia
⁴ Department of Infectious Disease and Microbiology, Imperial College, South Kensington Campus, London SW7 2AZ
⁵ TB Research Group, Veterinary Laboratories Agency - Weybridge, New Haw, Addlestone Surrey KT15 3NB
⁶ Armauer Hansen Research Institute, PO Box 1005, Addis Ababa, Ethiopia

In 2002, the prevalence of bovine tuberculosis (TB) among 500 cattle on Holeta Farm, near Addis Ababa, Ethiopia, was 48 per cent, and the farm was divided into positive and negative herds. After three consecutive rounds of skin testing and segregation of skin test-positive and -negative animals, the prevalence of bovine TB was reduced from 14 per cent to 1 per cent in the negative herd in a year. Spoligotyping of 41 isolates from 17 cows gave an identical and unique spoligotype pattern, which can be represented as the binary number 1100000101111110111111100010000000000100000, where 1 indicates the presence of a spacer and 0 represents a loss. This spoligotype pattern had not previously been reported on the Mycobacterium bovis spoligotype database, and it was therefore designated SB1176, Ethiopian M bovis strain 1 (EMbs1). The variable number tandem repeat (VNTR) profile of the strain was 5254^*33.1, which differed from the VNTR profile of strains reported in Great Britain.

This article has been cited by other articles:

				B. Muller, M. Hilty, S. Berg, M. C. Garcia-Pelayo, J. Dale, M. L. Boschiroli, S. Cadmus, B. N. R. Ngandolo, S. Godreuil, C. Diguimbaye-Djaibe, et al. African 1, an Epidemiologically Important Clonal Complex of Mycobacterium bovis Dominant in Mali, Nigeria, Cameroon, and Chad J. Bacteriol., March 15, 2009; 191(6): 1951 - 1960. [Abstract] [Full Text] [PDF]

				M. B. Boniotti, M. Goria, D. Loda, A. Garrone, A. Benedetto, A. Mondo, E. Tisato, M. Zanoni, S. Zoppi, A. Dondo, et al. Molecular Typing of Mycobacterium bovis Strains Isolated in Italy from 2000 to 2006 and Evaluation of Variable-Number Tandem Repeats for Geographically Optimized Genotyping J. Clin. Microbiol., March 1, 2009; 47(3): 636 - 644. [Abstract] [Full Text] [PDF]