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Detection of the genome of Chlamydophila abortus in samples taken from the uteri of 304 sheep at an abattoir

¹ Department of Veterinary Pathology, University of Liverpool, Leahurst, Neston, South Wirral LCH64 7TE
² Department of Obstetrics and Gynaecology, St George's Hospital Medical School, Cranmer Terrace, London SW17 0RE
³ Beredens Farm, Folkes Lane, Cranham, Essex RM14 1TH

A PCR was used to detect the genome of Chlamydophila abortus in samples of uterine tissue collected from 304 sheep by a sterile technique at an abattoir. The stage of pregnancy of the sheep was determined by measuring the dimensions of the embryo/fetus, and its morphology was recorded. Only samples from non-pregnant sheep and sheep up to 100 days of gestation were retained; the clinical history of the animals was unknown. The total prevalence of the chlamydial genome was 30·9 per cent, with a significantly higher prevalence in the pregnant animals (46·9 per cent). Higher detection rates were recorded during early gestation than during mid-gestation.

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				D. F. Twomey and P. C. Griffiths Detection of chlamydial DNA by PCR Vet Rec., September 29, 2007; 161(13): 460 - 460. [Full Text] [PDF]