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The Veterinary Record 161:153-155 (2007)
© 2007 British Veterinary Association

Papers and Articles

Detection of the genome of Chlamydophila abortus in samples taken from the uteri of 304 sheep at an abattoir

E. Michalopolou, DVM, PhD, MRCVS1, A. J. Leigh, BSc, PhD2 and L. G. Cordoba, DVM, MRCVS3

1 Department of Veterinary Pathology, University of Liverpool, Leahurst, Neston, South Wirral LCH64 7TE
2 Department of Obstetrics and Gynaecology, St George's Hospital Medical School, Cranmer Terrace, London SW17 0RE
3 Beredens Farm, Folkes Lane, Cranham, Essex RM14 1TH

A PCR was used to detect the genome of Chlamydophila abortus in samples of uterine tissue collected from 304 sheep by a sterile technique at an abattoir. The stage of pregnancy of the sheep was determined by measuring the dimensions of the embryo/fetus, and its morphology was recorded. Only samples from non-pregnant sheep and sheep up to 100 days of gestation were retained; the clinical history of the animals was unknown. The total prevalence of the chlamydial genome was 30·9 per cent, with a significantly higher prevalence in the pregnant animals (46·9 per cent). Higher detection rates were recorded during early gestation than during mid-gestation.




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J. A. Navarro, N. Ortega, A. J. Buendia, M. C. Gallego, C. M. Martinez, M. R. Caro, J. Sanchez, and J. Salinas
Diagnosis of placental pathogens in small ruminants by immunohistochemistry and PCR on paraffin-embedded samples
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D. F. Twomey and P. C. Griffiths
Detection of chlamydial DNA by PCR
Vet Rec., September 29, 2007; 161(13): 460 - 460.
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