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Experimental infection studies of UK Culicoides species midges with bluetongue virus serotypes 8 and 9

¹ Institute for Animal Health (IAH) Pirbright, Ash Road, Woking, Surrey GU24 0BN
² Centre for Applied Entomology and Parasitology, School of Life Sciences, Keele University, Staffordshire ST5 5BG
³ Institute of Biological and Environmental Sciences, University of Aberdeen, Tillydrone Avenue, Aberdeen AB24 2TZ

This paper describes a rapid, standardised method for testing the susceptibility to bluetongue virus (BTV) of northern Palaearctic Culicoides species midges that can be used to assess the competence of both field-caught and laboratory-infected midges. The method has been used to show that Culicoides scoticus can replicate BTV serotype 8 and BTV serotype 9 strains to more than 3 log₁₀ TCID50/midge, the first evidence of the potential of this species to transmit BTV.

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