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The Veterinary Record 164:655-658 (2009)
© 2009 British Veterinary Association

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Molecular characterisation of Mycobacterium bovis isolates from cattle carcases at a city slaughterhouse in Uganda

B. B. Asiimwe, BVM, MSc, PhD1, J. Asiimwe, BVM, MSc2, G. Kallenius, MD, PhD3, F. K. Ashaba, BSc1, S. Ghebremichael, BSc3, M. Joloba, MD, MS, PhD1 and T. Koivula, PhD4

1 Department of Medical Microbiology, Makerere University Medical School, PO Box 7072, Kampala, Uganda
2 Department of Veterinary Parasitology and Microbiology, Faculty of Veterinary Medicine, Makerere University, PO Box 7062, Kampala, Uganda
3 Department of Microbiology, Tumour and Cell Biology, Karolinska Institute, SE-171 77, Stockholm, Sweden
4 Department of Bacteriology, Swedish Institute for Infectious Disease Control, SE-171 82, Solna, Sweden

Correspondence: E-mail for correspondence: tuija.koivula{at}smi.se

During a period of eight months, the carcases of 16,800 slaughter cattle were inspected at a city abattoir in Uganda. Eighty-seven of them had tuberculosis-like lesions and tissue samples were cultured. Only 17 cultures yielded acid-fast bacilli; 11 of them were confirmed as Mycobacterium bovis and six as non-tuberculous mycobacteria (NTM). GenoType Mycobacterium assays on the six NTM identified two as Mycobacterium fortuitum and one as Mycobacterium intracellulare, but three were unidentified. Characterisation of the M bovis isolates by spoligotyping and IS6110 restriction fragment length polymorphism (RFLP) revealed that five of the six spoligotype patterns observed in the 11 strains had not been previously reported, and seven of the nine isolates typed by RFLP had multicopy number IS6110 patterns. Six of the 11 infected carcases had multiple sites of infection, but none was condemned as unfit for human consumption.






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