The Veterinary Record
HOME CURRENT ISSUE TABLE OF CONTENTS ARCHIVE SEARCH SUBSCRIPTIONS JOBS FEEDBACK HELP
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Navarro, J. A.
Right arrow Articles by Salinas, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Navarro, J. A.
Right arrow Articles by Salinas, J.
The Veterinary Record 165:175-178 (2009)
© 2009 British Veterinary Association

Papers

Diagnosis of placental pathogens in small ruminants by immunohistochemistry and PCR on paraffin-embedded samples

J. A. Navarro, DVM, PhD, DipECVP1, N. Ortega, DVM, PhD2, A. J. Buendia, DVM, PhD1, M. C. Gallego, DVM, PhD2, C. M. Martínez, DVM, PhD1, M. R. Caro, DVM, PhD2, J. Sánchez, DVM, PhD, DipECVP1 and J. Salinas, DVM, PhD2

1 Departamento de Anatomía y Anatomía Patológica Comparadas, Facultad de Veterinaria, Universidad de Murcia, Campus de Espinado, Murcia 30100, Spain
2 Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad de Murcia, Campus de Espinado, Murcia 30100, Spain

Correspondence: E-mail for correspondence: jnavarro{at}um.es

A histological study was carried out on 58 formalin-fixed and paraffin-embedded samples of placenta from sheep and goats that had aborted, and the placental lesions were graded. Sequential histological sections of each cotyledon were then immunostained with specific antibodies and used for PCR detection of Chlamydophila abortus, Coxiella burnetii, Salmonella Abortusovis, Brucella melitensis, Listeria monocytogenes and Toxoplasma gondii. Most of the cotyledons showed different degrees of placentitis. The proportional agreement between the two techniques was 0·879 ({kappa} value 0·746). C abortus was the most prevalent pathogen. Mixed infections were common.






HOME CURRENT ISSUE TABLE OF CONTENTS ARCHIVE SEARCH SUBSCRIPTIONS JOBS FEEDBACK HELP
Copyright © 2009 British Veterinary Association